rabbit polyclonal anti jagged1 antibody  (Danaher Inc)

Journal: Neural Regeneration Research

Article Title: Astrocyte-neuron communication mediated by the Notch signaling pathway: focusing on glutamate transport and synaptic plasticity

doi: 10.4103/1673-5374.369124

Figure Lengend Snippet: Effects of HI on Notch1 and Jagged1 expression and Notch1/Jagged1 double-positive immunofluorescence in the hippocampal region in pigs after HI. (A) Notch1/Jagged1 double immunofluorescence staining in the brain of the control group and of the HI groups 0–6, 6–12, 12–24, and 24–48 hours after injury (red fluorescence represents Notch1, green fluorescence represents Jagged1, and blue fluorescence represents nuclei) (scale bar: 20 μm). Images are representative of three other images. (B) Change in mean optical density of Notch1 protein expression. In the 0–6 hours post-HI group, Notch1 expression was decreased, whereas it was increased in the 12–24 hours post-HI group. (C) Change in mean optical density of Jagged1 protein expression. In the 12–24 hours post-HI group, Notch1 expression was increased compared with the 6–12 hours group, whereas it was decreased in the 24–48 hours post-HI group. (D) Change in the percentage of Notch1/Jagged1 double-positive cells. The percentage of Notch1/Jagged1 double-positive cells was decreased at 0–6 hours, increased at 12–24 hours, and decreased again at 24–48 hours after HI. Data are expressed as the mean ± SEM. * P < 0.05, ** P < 0.01 (one-way analysis of variance followed by Tukey’s post hoc test for B and C; one-way analysis of variance followed by Dunnett’s t -test for D). All experiments were repeated three times. HI: Hypoxic ischemia.

Article Snippet: The primary antibodies used for immunofluorescence staining and their dilutions were as follows: mouse monoclonal anti-Notch1 antibody (1:50, Abcam, Cat# ab44986, RRID: AB_776840); rabbit polyclonal anti-Jagged1 antibody (1:50, Abcam, Cat# ab7771, RRID: AB_2280547); rabbit polyclonal anti-EAAT2 antibody (1:50, Abcam, Cat# ab203130, RRID: AB_2924794); and rabbit polyclonal anti-synaptophysin antibody (1:100, Abcam, Cat# ab14692, RRID: AB_301417).

Techniques: Expressing, Immunofluorescence, Double Immunofluorescence Staining, Control, Fluorescence

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Regulation of γδT17 cells by Mycobacterium vaccae through interference with Notch/Jagged1 signaling pathway

doi: 10.1590/1414-431X20209551

Figure Lengend Snippet: Expression of Jagged1 using immunohistochemical and immunofluorescence stains ( A ) in mice in control group ( a ), asthma group ( b ), gamma-secretase inhibitor (DAPT) + ovalbumin (OVA) group ( c ), Mycobacterium vaccae + OVA group ( d ), OVA + M. vaccae group ( e ), and its quantification ( B and C ). Data are reported as mean±SE relative percentage to the control group (n=8). ## P<0.01 vs control; **P <0.01 vs asthma group (ANVOA). Original magnification x400, scale bar=50 μm.

Article Snippet: The tissue sections were incubated in 5% goat serum albumin and subsequently in polyclonal goat anti-human Jagged1 antibody (1:2500; D4Y1R, CST) for 24 h at 4°C,Then, the tissue sections were incubated with the horseradish peroxidase-conjugated secondary antibody for 60 min at room temperature, and subsequently stained with 3,3-diaminobenzidine and counterstained with hematoxylin blue.

Techniques: Expressing, Immunohistochemical staining, Immunofluorescence, Control

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Regulation of γδT17 cells by Mycobacterium vaccae through interference with Notch/Jagged1 signaling pathway

doi: 10.1590/1414-431X20209551

Figure Lengend Snippet: The mRNA expression of Jagged1 in lung tissues was determined using real-time PCR. Data are reported as mean±SE relative percentage to the control group (n=8). *P<0.05 vs control; # P<0.05 vs asthma group (ANOVA). OVA: ovalbumin; DAPT: gamma-secretase inhibitor.

Article Snippet: The tissue sections were incubated in 5% goat serum albumin and subsequently in polyclonal goat anti-human Jagged1 antibody (1:2500; D4Y1R, CST) for 24 h at 4°C,Then, the tissue sections were incubated with the horseradish peroxidase-conjugated secondary antibody for 60 min at room temperature, and subsequently stained with 3,3-diaminobenzidine and counterstained with hematoxylin blue.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Regulation of γδT17 cells by Mycobacterium vaccae through interference with Notch/Jagged1 signaling pathway

doi: 10.1590/1414-431X20209551

Figure Lengend Snippet: Correlation analysis between Jagged1 and γδT17 cells in lung tissues. A and B , The ratio of IL-17+γδT+ cells to T cells was measured in each group. C , The percentage of IL-17+γδT+ cells and the Jagged1 mRNA expression in lung tissues were positively correlated (r=0.46, P<0.05). Data are reported as mean±SE relative percentage to the control group (n=8). **P<0.01 vs control; *P<0.05, ## P<0.01 vs asthma group (ANOVA). OVA: ovalbumin; DAPT: gamma-secretase inhibitor.

Article Snippet: The tissue sections were incubated in 5% goat serum albumin and subsequently in polyclonal goat anti-human Jagged1 antibody (1:2500; D4Y1R, CST) for 24 h at 4°C,Then, the tissue sections were incubated with the horseradish peroxidase-conjugated secondary antibody for 60 min at room temperature, and subsequently stained with 3,3-diaminobenzidine and counterstained with hematoxylin blue.

Techniques: Expressing, Control